Turbovet Bovine ITIH4
Turbidimetric test for the determination of bovine ITIH4
Fast, automatic, precise, robust: All the advantages of a turbidimetric method with the reliability of a species-specific test. Test ITIH4 to assess animal health and welfare or quantify inflammation.
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Uses and advantages of our test
Turbovet bovine ITIH4 is the first commercially available method for the determination of bovine ITIH4 based on turbidimetry. As a bovine acute-phase protein, ITIH4 can be used for diagnosis and monitoring of infection or inflammatory processes. The ITIH4 response is slower but longer lasting compared with haptoglobin in bovine species. Combinatorial analysis of both biomarkers is ideal for highly sensitive detection of health problems or poor animal welfare on livestock farms. The Turbovet method offers fully automated analysis using any open clinical chemistry analyzer, minimizing the required time and cost. Turbovet is precise and reproducible, and provides results in a few minutes. The reagents are very stable and frequent calibration is not necessary. Turbovet is an ideal analytical tool for quality control in production systems, animal-welfare certification, or studies requiring highly reliable and reproducible data.
TECHNICAL DATA
• Species-specific
• Automatic: Can be run in any open clinical chemistry analyzer.
• Working range: 0-4 mg/mL .
• Assay time: 5 minutes
• Reagents: 2 reagents ready to use
• No interference by hemoglobin (20g/L), bilirubin (0.15 g/L) or triglycerides (10g/L)
• Sample type: serum
ANALYTICAL PRINCIPLE:
Quantification by immunoturbidimetry. ITIH4 from serum reacts with specific antibodies to form immunoaggregates. This causes an increase in turbidity in the reaction medium. The increase in turbidity is determined by photometric measurement and is proportional to ITIH4 concentration in the sample.
STANDARDIZATION:
The assay is calibrated with a bovine-specific calibrator, whose value is adjusted with an internal standard. There is not international reference material for bovine ITIH4.
VALIDATION:
Validated by the Autonomous University of Barcelona according to CLSI and SEQC guidelines.
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