(+34) 976 731 533 info@acuvetbiotech.com

Turbovet Bovine Hp

Turbidimetric test for the determination of bovine haptoglobin

Fast, automatic, precise, robust: All the advantages of a turbidimetric method with the reliability of a bovine-specific test, also valid for hemolyzed samples. Testing haptoglobin provides insight in health-and-welfare status of your animals and enables quantification of inflammatory response.

Available formats

CATALOG NR
AC/BHPT01
AC/BHPST
AC/BHPCT
DESCRIPTION
Turbovet bovine Hp, complete kit. Includes calibrator and controls.
Turbovet bovine Hp calibrator.
Turbovet bovine Hp controls.

Uses and advantages of our test

Haptoglobin is a major acute-phase protein in cattle. It is a very sensitive marker for the detection of infections or inflammatory processes, even subclinical conditions, as well as stress. In cattle, basal haptoglobin levels are low, and the response of the biomarker is rapid and correlated with the severity of disease or the degree of stress. Analysis can enable early disease detection, and evaluation of the efficacy of treatments. It also provides a quantitative and objective measure of the on-farm health-and-welfare status of animals. Turbovet is the first turbidimetric method for haptoglobin analysis, specific to bovine species. It is a fast method with results in a few minutes, and can be automated with any open clinical chemistry analyzer. The method is sufficiently sensitive to measure basal levels, and offers very precise and reproducible results. Unlike the colorimetric method, our turbidimetric test is not affected by hemolysis, making this an ideal tool for quality control in production systems, animal-welfare certification, and in clinical diagnostics laboratories, where this biomarker can be easily incorporated into analytical profiles.

TECHNICAL DATA

• Species-specific
• Automatic: Can be run in any open clinical chemistry analyzer.
• Working range: 0-400 mg/L
• Assay time: 5 minutes
• Reagents: 2 reagents, ready to use.
• No interferences by hemoglobin (20g/L) bilirubin (0.15 g/L) or triglycerides (10g/L).
• Type of sample: serum.

ANALYTICAL PRINCIPLE

Quantification by particle-enhanced immunoturbidimetry. Hp from serum reacts with specific antibodies fixed to latex microparticles, forming aggregates that originate an increase in turbidity in the reaction medium. The increase in turbidity is determined by photometric measurement and is proportional to Hp concentration in the sample.

STANDARDIZATION:

The assay is calibrated with a bovine-specific calibrator, according to the European Reference Serum for Bovine Acute Phase Proteins (EU concerted Action QLK5-CT-1999-0153).

VALIDATION:

Validated by the Autonomous University of Barcelona according to CLSI and SEQC guidelines.

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